产品描述
Products Descriptions
牛冠状病毒（BCV）单克隆抗体（BC 6-4）♦ BC 6-4 与牛冠状病毒（BCV）的北美株反应。该单克隆抗体识别 BCV 核衣壳蛋白上的一个表位。该抗体以小鼠腹水形式生产。现有一种 BC 6-4 产品：BC 6-4-A（未偶联腹水）。亚型：IgG1。BC 6-4 的用途：IHC、IFA、ELISA
猪繁殖与呼吸综合征病毒（PRRSV）单克隆抗体（AT13-A）♦ 单克隆抗体 AT-13 识别猪繁殖与呼吸综合征病毒（PRRSV）核衣壳蛋白内一个高度保守的、构象依赖性表位。它是针对最近在美国分离的类欧洲株（美国 I 型）制备的。它与 PRRSV 的欧洲株、类欧洲株和北美分离株具有高度的交叉反应性。该单克隆抗体以小鼠腹水形式生产。现有一种 AT13 产品：AT13-A（未偶联腹水）。亚型：IgM。AT-13 产品推荐用于免疫沉淀、间接荧光抗体和酶联免疫吸附测定。AT-13 不识别蛋白质印迹中的蛋白质。
猪繁殖与呼吸综合征病毒（PRRSV）单克隆抗体（SDOW17）♦ SDOW17 与超过 99% 的猪繁殖与呼吸综合征病毒（PRRSV）北美株和欧洲株反应。该单克隆抗体识别 PRRSV 核衣壳蛋白上一个高度保守的表位。SDOW17 不与 Prime Pac PRRSV 疫苗株反应。该抗体以小鼠腹水形式生产。现有两种 SDOW17 产品：SDOW17-A（未偶联腹水）和 SDOW17-F（与异硫氰酸荧光素（FITC）偶联的腹水）。亚型：IgG1。用途及推荐稀释度 ——SDOW17-A：IFA—1:50,000，IHC—1:10,000；SDOW17-F：IFA—1:100。
猪繁殖与呼吸综合征病毒（PRRSV）单克隆抗体（SR30）♦ SR30 基本上与所有猪繁殖与呼吸综合征病毒（PRRSV）的北美株和欧洲株反应。该单克隆抗体识别 PRRSV 核衣壳蛋白上一个高度保守的表位。该抗体以小鼠腹水形式生产。现有两种 SR30 产品：SR30-A（未偶联腹水）和 SR30-F（与异硫氰酸荧光素（FITC）偶联的腹水）。亚型：IgG1。用途及推荐稀释度 ——SR30-A：IFA—1:50,000，IHC—1:10,000；SR30-F：IFA—1:100。
大肠杆菌 K88ac 单克隆抗体（EC K88）♦ 抗 K88ac 单克隆抗体是针对产肠毒素大肠杆菌（ETEC）的 K88 菌毛制备的。该抗体与 ETEC 的 K88 菌毛反应，并阻断 K88 + 细菌与肠上皮细胞的结合。该抗体在杂交瘤细胞中生产，以细胞培养上清液形式提供。亚型：IgG1。EC K88 的用途：IFA。推荐稀释度：1:100。
轮状病毒（RV）单克隆抗体（RV 11-2）♦ RV 11-2 与轮状病毒（RV）的 A 组毒株反应。该单克隆抗体识别 RV 的 VP6 蛋白上的一个表位。该抗体以小鼠腹水形式生产。现有一种 RV 11-2 产品：RV 11-2-A（未偶联腹水）。亚型：IgG2a。RV 11-2 的用途：RV11-2-A：IFA、ELISA。
猪圆环病毒单克隆抗体（PCV2）♦ PCV2 与猪圆环病毒 2 型反应。该抗体以小鼠腹水形式生产。现有一种 PCV2 产品：PCV2-A（未偶联腹水）。亚型：IgG2。用途及推荐稀释度：IFA—1:1000。
偶联信息（请咨询可用性）
抗体可偶联至不同标记物，包括酶、生物素和荧光染料 / 蛋白。所选标记物主要取决于实验应用。
异硫氰酸荧光素（FITC）是一种荧光染料，可吸收紫外光或蓝光，使分子激发并发出可见的黄绿色光。移除激发光后，发光停止。当反应物之一是细胞、组织或其他生物结构的一部分时，荧光标记可快速、准确地定位抗原 - 抗体相互作用。FITC 是免疫荧光技术中常用的抗体标记物，因为 FITC 与蛋白质的偶联相对容易，且通常不会破坏标记蛋白的生物学活性。FITC 广泛用作半抗原以标记不同蛋白质。抗 FITC 的抗体用于识别 FITC 标记的蛋白质，并作为模型研究抗体对明确半抗原的应答机制。
当目标靶标表达水平低，仅使用标记的二抗无法检测时，生物素是常用的标记物。多个生物素分子可偶联至一个二抗。
酶是蛋白质，其作用的分子称为底物。通过显色反应（借助酶组织化学方法）可使酶标记可视化，其中可溶性无色底物转化为水不溶性有色化合物。常用的酶之一是辣根过氧化物酶（HRP）。
辣根过氧化物酶（HRP）可通过化学发光等显色反应或使用二氨基联苯胺（DAB）进行可视化。HRP 是一种 44kDa 的糖蛋白酶标记物，比碱性磷酸酶更稳定。
Bovine Coronavirus (BCV) Monoclonal Antibody (BC 6-4) ♦ BC 6-4 reacts with North American strains of Bovine Coronavirus (BCV). This monoclonal antibody recognizes an epitope on the nucleocapsid protein of BCV. The antibody is produced as mouse ascites fluid. One BC 6-4 product is available: BC 6-4-A (unconjugated ascites fluid). Isotype-IgG1. Uses of BC 6-4-IHC, IFA, ELISA
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Monoclonal Antibody (AT13-A) ♦ Monoclonal antibody AT-13 recognizes a highly conserved, conformational dependent epitope within the nucleocapsid protein of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). It was produced against a European-like strain (U.S. type I) recently isolated within the United States. It has a high degree of cross reactivity to European, European like, and North American isolates of PRRSV. This monoclonal antibody is produced as mouse ascites fluid. One AT13 product is available: AT13-A (unconjugated ascites fluid). Isotype: IgM. Product AT-13 is recommended for use in immunoprecipitation, indirect fluorescent antibody, and enzyme linked-immunosorbent assay applications. AT-13 does not recognize proteins on Western blot.
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Monoclonal Antibody (SDOW17) ♦ SDOW17 reacts with >99% of North American and European isolates of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). This monoclonal antibody recognizes a highly conserved epitope on the nucleocapsid protein of PRRSV. SDOW17 does not react with the Prime Pac PRRSV vaccine strain. This antibody is produced as mouse ascites fluid. Two SDOW17 products are available: SDOW17-A (unconjugated ascites fluid) and SDOW17-F (ascites fluid conjugated with fluorescein isothiocyanate {FITC}). Isotype-IgG1. Uses and Recommended Dilutions – SDOW17-A: IFA—1:50,000, IHC—1:10,000. SDOW17-F: IFA—1:100
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Monoclonal Antibody (SR30) ♦ SR30 reacts with essentially all North American and European strains of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). This monoclonal antibody recognizes a highly conserved epitope on the nucleocapsid protein of PRRSV. The antibody is produced as mouse ascites fluid. Two SR30 products are available: SR30-A (unconjugated ascites fluid) and SR30-F (ascites fluid conjugated with fluorescein isothiocyanate {FITC}). Isotype-IgG1. Uses and Recommended Dilutions – SR30-A: IFA—1:50,000, IHC—1:10,000, SR30-F: IFA—1:100
Escherichia coli K88ac Monoclonal Antibody (EC K88) ♦ The anti-K88ac monoclonal antibody was created against the K88 fimbriae of enterotoxigenic Escherichia coli (ETEC). The antibody reacts with K88 fimbriae of ETEC and blocks the binding of K88+ bacteria to intestinal epithelial cells. The antibody is produced in hybridoma cells and is available as cell culture supernatant. Isotype–IgG1. Uses of EC K88-IFA. Recommended Dilution—1:100
Rotavirus (RV) Monoclonal Antibody (RV 11-2) ♦ RV 11-2 reacts with Group A strains of rotavirus (RV). This monoclonal antibody recognizes an epitope on the VP6 protein of RV. The antibody is produced as mouse ascites fluid. One RV 11-2 product is available: RV 11-2-A (unconjugated ascites fluid). Isotype-IgG2a. Uses of RV 11-2: RV11-2-A: IFA, ELISA.
Porcine Circovirus Monoclonal Antibody (PCV2) ♦ PCV2 reacts with porcine circovirus 2 virus. The antibody is produced as mouse ascites fluid. One PCV2 product is available: PCV2-A (unconjugated ascites fluid). Isotype-IgG2. Uses and Recommended Dilution, IFA—1:1000
Conjugation Information (Please Inquire for Availability)
Antibodies can be conjugated to different labels, including enzymes, biotin, and fluorescent dyes/proteins. The label of choice essentially depends upon the experimental application.
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light, causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue, or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques because the conjugation of FITC to proteins is relatively easy and generally does not destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Antibodies to FITC are used to identify FITC labeled proteins and as models to study the mechanism of antibody response to a well-defined hapten.
Biotin is commonly used when the target of interest is expressed at low levels and cannot be detected using secondary labeled antibodies alone. Many biotin molecules can be conjugated to a secondary antibody.
Enzymes are proteins, and the molecules upon which they act are known as substrates. The enzyme label can be visualized by means of enzyme histochemical methods via chromogenic reactions, wherein a soluble colorless substrate is converted to a water-insoluble colored compound. One of the more commonly used enzymes is Horse radish peroxidase (HRP).
Horse radish peroxidase (HRP) can be visualized by chromogenic reactions such as chemiluminescence or through the use of diaminobenzidine (DAB). HRP is a 44kDa glycoprotein enzyme label and is more stable than alkaline phosphatase.
